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Multidrug-resistant (MDR) strains of Mycobacterium tuberculosis represent an obstacle in tuberculosis control and stress the need for rapid and reliable diagnostic tools for drug susceptibility testing in clinical isolates. Mutations in the 81-bp region of the rpoB gene are responsible for resistance to rifampicin, an essential drug for the therapy of tuberculosis (TB). Mycobacteria laboratory facilities in Sudan exist only in Khartoum state whereas TB patients from other states are referred to Khartoum state for diagnosis and treatment. The MDR-TB prevalence in Sudan was found in 5% of new cases and 24% of previously treated patients. Recent development of the Xpert MTB/RIF test and of the line probe assay which span 81-bp fragment of the RNA polymerase beta subunit (rpoB) gene have allowed for the rapid detection of resistance to rifampicin (RIF). Knowledge on the geographical distribution of the rpoB resistant alleles is essential for the development of diagnostic tools. Although sequence analysis of the rpoB gene has been shown to be effective for detecting RIF-resistance alleles in over 90% of RIF-resistant isolates from different geographical regions, there is only limited information from Sudan. In order to find Sudan-specific mutations, that could potentially be used for the optimization of the current detection tools of RIF resistance alleles, we determined in this study the mutation profile occurring within an 81-bp fragment of the rpoB gene, in a collection of MDR clinical isolates of Mycobacterium tuberculosis isolated from Khartoum, Sudan.

Palabras clave: rpoB; tuberculosis; TB; Sudan.

2014-06-13   |   421 visitas   |   Evalua este artículo 0 valoraciones

Vol. 8 Núm.6. Junio 2014 Pags. 796-798 J Infect Developing Countries 2014; 8(6)