CSF RT-PCR standardization in pediatric patients with enteroviral central nervous system infection

Autores: García Elorriaga Guadalupe, Esparza García Amalia, Méndez Rojas Claudia, Del Rey Pineda Guillermo, González Bonilla César R.

Resumen

Objective: We standardized the RT-PCR panviral CSF and determined its applicability in detecting acute enterovirus infection in the central nervous system in children under 15 years. Material and methods: RT-PCR was performed directly in CSF samples of 10 pediatric patients with suspected CNS infection and 9, with different conditions of the central nervous system. Results: 80% (8/10) of RT-PCR samples were positive for enterovirus in patients with suspected CNS infection and no sample was positive in patients with different ailments. Conclusions: Since enteroviruses are among the main etiologies of pediatric encephalitis, RT-PCR could be particularly useful for rapid detection in CSF.

Palabras clave: Enterovirus RT-PCR infection of the CNS.

2014-11-06   |   288 visitas   |   Evalua este artículo 0 valoraciones

Vol. 64 Núm.1. Enero-Enero 2012 Pags. 59-66 Rev Invest Clin 2012; 64(1-ENGLISH)