Resumen

Introduction: Rapid dissemination of plasmid-mediated quinolone resistance (PMQR) has been reported in clinical isolates. Methodology: A total of 149 clinical isolates of Enterobacteriaceae were collected in Beijing and screened for PMQR genes usingpolymerase chain reaction (PCR). Real-time quantitative PCR was used to study the expression of qnrS. Results: The rates of qnr and aac(6’)-Ib-cr genes were 7.4% and 8.1%, respectively. The higher basal expression of qnrS was observed in transconjugant strains, which had higher minimum inhibitory concentrations (MICs) of quinolones. Furthermore, qnrS expression levels increased in all three isolates when a quinolone was present. Conclusions: Our data suggest that the level of qnrS expression was associated with quinolone resistance.

Palabras clave: Enterobacteriaceae; PMQR; qnrS; expression.

2014-12-15   |   337 visitas   |   Evalua este artículo 0 valoraciones

Vol. 8 Núm.12. Diciembre 2014 Pags. 1625-1629 J Infect Developing Countries 2014; 8(12)