Resumen

Objective: To characterize a neuron-enriched primary TG culture and evaluate interferón-â expression and activity after HSV-1 infection. Materials and methods: The percentage of neurons present in cultures was assessed by neurofilament immunocytochemistry. Cultures were treated with interferón-â and infected with HSV-1, then viral antigen positive cells were counted and interferón-â expression was assessed by quantitative PCR. Results: The culture contained 15% neurons and 85% non-neuronal cells. A cytopathic effect was observed, associated with high viral spread (72.9% neurons and 48.3% non-neuronal cells were positive for viral antigen).Interferón-â treatment impaired the cytopathic effect and decreased the infected neurons to 16.7% and infected non-neuronal cells to 7.8%. Viral infection at 6 h post- infection significantly increased the interferón-â transcripts by 18.2 fold, while at 18 h post-infection Interferon pre-treatment in infected cultures increased interferón-â transcription by 3.7 fold. Discussion: This culture model contained 15% neurons, which is 10 times higher compared to other reported cultures, and non-neuronal cells comprised 85% of cells in this culture. All types of cells were found to be infected, which is similar to that reported during acute infections in vivo. Additionally, interferón-â decreased the infected cells, avoiding the cytopathic effect, which is similar to that reported in swine TG cultures. Conclusions: A neuron-enriched primary TG model was characterized. interferón-â treatment protected cells from cytopathic effects and viral spread, while viral infection up-regulated interferón-â expression. This result means that interferón-â exerts an important antiviral effect against HSV-1 in these cultures.

Palabras clave: HSV-1 interferón-â trigeminal ganglion neurons neurofilament.

2017-03-20   |   132 visitas   |   Evalua este artículo 0 valoraciones

Vol. 18 Núm.2. Abril-Junio 2014 Pags. 37-44 Infectio 2014; 18(2)