Fragmento

A strong reactivity was observed in the ZP with the lectins; however, no labelling was detected with the antibodies against sialyl-lewis epitopes. The sperm-ZP binding was 73% and 81% inhibited with LFA and MAA, respectively. This inhibition was dose dependent. No effect was observed with the SNA lectin. A 60% and 95% inhibition of the sperm binding to the ZP was observed when the ZP was treated with the neuraminidase at 1U/ml and 10U/ml, respectively. A 81% inhibition of fertilization was detected in IVF experiments using oocytes previously incubated with neuraminidase. Only neuraminidase activity was detected in the oocyte. This activity was mainly associated with the oocyte exudates obtained alter activation with the calcium ionophore.

Palabras clave: Sialic acid neuraminidase lectins sperm-zp interaction bovine.

2009-06-02   |   654 visitas   |   Evalua este artículo 0 valoraciones

Vol. 8 Núm.1. Enero-Junio 2004 Pags. 24. Rev Orinoquia 2004; 8(1)