Prevalence of enterotoxigenic and Shiga toxin-producing Escherichia coli in pigs slaughtered in Mato Grosso, Brazil

Autores: Prado Martins Rodrigo, da Silva Maria Cristina, Dutra Valéria, Nakazato Luciano, da Silva Leite Domingos

Resumen

Introduction: This study aimed to estimate the prevalence of enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing Escherichia coli (STEC) strains in pigs slaughtered in abattoirs located in the state of Mato Grosso, Brazil. Methodology: Intestinal samples from 74 animals were aseptically dissected and lumen content was plated on MacConkey agar. Confluent colonies from each plate were screened for the presence of ETEC and STEC strains by PCR assays. Results: It was verified that the prevalence of STEC and ETEC carriers was 1.35% and 9.46% respectively. One (1.35%) of the 74 samples tested was positive for the stx2 gene, and seven (9.46%) for st1, of which two (2.70%) were also positive for lt1. Conclusion: The results provided represent a benchmark for future research on pathogenic E. coli of porcine origin in Mato Grosso.

Palabras clave: STEC ETEC PCR slaughterhouse Mato Grosso.

2011-04-18   |   369 visitas   |   Evalua este artículo 0 valoraciones

Vol. 5 Núm.2. Febrero 2011 Pags. 123-126. J Infect Developing Countries 2011; 5(2)