Resumen

Background: The emerging trends of multidrug resistance among several groups of microorganisms against different classes of antibiotics led different researchers to develop efficient drugs from plant sources to counter multidrug resistant strains. This study investigated different solvent extracts of Prosopis spicigera (P. Spicigera), Zingiber officinale, and Trachyspermum ammi (T. ammi) to determine their efficacy against multidrug resistant microbes. Methodology: Successive extractions of these plants were performed using a Soxhlet apparatus, using solvents with increasing polarities. Preliminary phytochemical analysis was also performed .Minimum inhibitory concentration was determined by a two-fold serial dilution method followed by determination of minimum bactericidal/fungicidal concentration. Multidrug resistant (MDR) strains of Candida albicans, Candida krusei, Candida tropicalis, Candida glabrata, Escherichia coli and reference strains of Streptococcus mutans and Streptococcus bovis were used in the study. Results: The ethanolic fraction of P. spicigera (least minimum inhibitory concentration [MIC] - 4.88 μg/ml) demonstrated a remarkable inhibition of the microorganisms while fractions obtained from those of Zingiber officinale (least MIC-78.125 μg/ml) exhibited little activity. The petroleum ether fraction of T. ammi (least MIC- 625 μg/ml) showed best activity when compared to its other fractions. Qualitative analysis of the phytoconstituents was also performed. Conclusions: The potency shown by these extracts recommends their use against multidrug resistant microorganisms. This study also showed that P. spicigera could be a potential source of new antimicrobial agents.

Palabras clave: Multidrug resistance ESBLs plant extracts bacteria fungus.

2011-04-20   |   670 visitas   |   Evalua este artículo 0 valoraciones

Vol. 4 Núm.5. Mayo 2010 Pags. 292-300. J Infect Developing Countries 2010; 4(5)