Resumen

Misidentification of Brucella species fron clinical specimens using commercial bacterial identification systems is a recurring problem. An isolate fron a bacterimic patient was identified as Bergeyella zoohelcum by MicroScan Walk-Away (Siemens Healthcare Diagnostics Inc., West Sacramento, CA, USA) and as Brucella melitensis by Vitek 2 system (bioMérieux Inc., Durham, NC, USA). Because of this identification ambiguity by the two automated bacterial identification systems we performed 16S rRNA sequencing and serotyping of the isolate and confirmed it as a Brucella spp. Combining the sequence data with the Vitek 2 system data we conclude that the infection was caused by B. melitensis.

Palabras clave: 16S rRNA sequencing misidentification Brucella melitensis Bergeyella zoohelcum automated bacterial identification systems.

2012-03-16   |   495 visitas   |   Evalua este artículo 0 valoraciones

Vol. 6 Núm.3. Marzo 2012 Pags. 283-286 J Infect Developing Countries 2012; 6(3)