Resumen

Materials and methods: Four interactional treatments were implemented between two freezing (rapid and slow) and two thawing (rapid and slow) curves, in a 2 x 2 factorial as follows: rapid freezing-rapid thawing, rapid freezing-slow thawing, slow freezing-rapid thawing, and slow freezing-slow thawing. After thawing by Sperm Class Analyzer (SCA) curvilinear velocity (VCL) and straight-line (VSL) (µm sec^-1) were analyzed; total, rapid, medium, and slow motility, were compared among treatments. Results: The rapid freezing-slow thawing treatment was lethal for all variables of velocity and motility, causing a significant(p < 0.01) post-thaw inmotility of 100%. The slow freezing-rapid thawing interaction had a significantlyhigher effect than the other treatments (p < 0.05), particularly on variables such as rapid motility (10.1 ± 1.1%), medium motility (30.16 ± 4.1%), and curvilinear velocity (51.5 ± 4.75 ìm sec.-1) also decreased the percentage of sperm with slow motility (41.7 ± 4.45%). Independently of the applied thawing rate, the freezing rate generated the main significanteffect on total motility. Conclusions: It is possible to conclude that the interaction effect between freezing and thawing rates is nil (except for slow motility) during cryopreservation process. However, the independent effects of these factors (main effects) on remaining motility variables are positively significantand decisive to the maintenance of these features, especially the freeze factor (when it is slow). This becomes the firstsuccessful report of sperm cryopreservation from Bocachico Prochilodus magdalenae in the world and may be used in conservation programs for this endangered species.

Palabras clave: Cryopreservation freezing motility sperm thawing (Source:CAB).

2013-04-10   |   318 visitas   |   Evalua este artículo 0 valoraciones

Vol. 17 Núm.3. Septiembre-Diciembre 2012 Pags. 3295-3303 Rev MVZ Córdoba 2012; 17(3)