Resumen

A simple, precise and rapid high-performance thin-layer chromatographic method has been developed for the estimation of phyllanthin and is the important lignans of Phyllanthus amarus. Separation of phyllanthin was carried out on silica gel 60 F₂₅₄ layers eluted with hexane: ethyl acetate (2:1), and the analytes were visualized through colour development with 10% concentrated sulphuric acid in ethanol. Scanning and quantification of spots was performed at 200 nm. The proposed method being precise and sensitive can be used for the detection, monitoring and quantification of phyllanthin from Phyllanthus amarus.

Palabras clave: HPTLC Phyllanthus amarus Phyllanthin Lignans.

2013-09-10   |   276 visitas   |   Evalua este artículo 0 valoraciones

Vol. 9 Núm.5. Septiembre 2010 Pags. 353-358 BLACPMA 2010; 9(5)